期刊
REGENERATIVE MEDICINE
卷 11, 期 6, 页码 545-557出版社
FUTURE MEDICINE LTD
DOI: 10.2217/rme-2016-0037
关键词
cytokines gene ontology analysis; immunology biotechnology; psoriasis transcriptome; skin gene expression profiling; tissue engineering immune response
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canadian Institutes of Health Research (CIHR) through their joint Collaborative Health Research Projects (CHRP) program [MOP-311262]
- 'Fonds d'Enseignement et de Recherche' (FER) of the Faculte de Pharmacie, Universite Laval, Quebec, Canada
- Reseau TheCell du FRQS
Aim: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used. Materials & methods: Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-alpha, IL-1 alpha, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray. Results: Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU). The expression of those genes was dramatically increased compared with basal levels of controls (p < 0.005 to < 0.05). Conclusion: Psoriatic substitutes supplemented with a cocktail of TNF-alpha, IL-1 alpha, IL-6 and IL-17A showed similar transcriptome alterations to those found in psoriasis.
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