4.3 Article

Tissue-engineered human psoriatic skin supplemented with cytokines as an in vitro model to study plaque psoriasis

期刊

REGENERATIVE MEDICINE
卷 11, 期 6, 页码 545-557

出版社

FUTURE MEDICINE LTD
DOI: 10.2217/rme-2016-0037

关键词

cytokines gene ontology analysis; immunology biotechnology; psoriasis transcriptome; skin gene expression profiling; tissue engineering immune response

资金

  1. Natural Sciences and Engineering Research Council of Canada (NSERC)
  2. Canadian Institutes of Health Research (CIHR) through their joint Collaborative Health Research Projects (CHRP) program [MOP-311262]
  3. 'Fonds d'Enseignement et de Recherche' (FER) of the Faculte de Pharmacie, Universite Laval, Quebec, Canada
  4. Reseau TheCell du FRQS

向作者/读者索取更多资源

Aim: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used. Materials & methods: Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-alpha, IL-1 alpha, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray. Results: Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU). The expression of those genes was dramatically increased compared with basal levels of controls (p < 0.005 to < 0.05). Conclusion: Psoriatic substitutes supplemented with a cocktail of TNF-alpha, IL-1 alpha, IL-6 and IL-17A showed similar transcriptome alterations to those found in psoriasis.

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