期刊
RADIOLOGY
卷 280, 期 1, 页码 137-150出版社
RADIOLOGICAL SOC NORTH AMERICA (RSNA)
DOI: 10.1148/radiol.2016160191
关键词
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资金
- Damon Runyon Cancer Research Foundation [DRR-29-14]
- Pershing Square Sohn Cancer Research Alliance
- RSNA Research Scholar Grant
- MSKCC Center for Molecular Imaging and Nanotechnology Grant
- Commonwealth Foundation for Cancer Research
- Center for Experimental Therapeutics Center of Memorial Sloan Kettering Cancer Center
- MSKCC Technology Development Grant
- Geoffrey Beene Cancer Research Center Grant Award
- Society of MSKCC Research Grant
- Deutsche Forschungsgemeinschaft (DFG) [NE 1922/2-1]
- Heinrich Hertz-Stiftung Research Fellowship Grant
- NIH/NCI Cancer Center Support Grant [P30 CA008748]
- National Institutes of Health [R01 EB017748, K08 CA16396, 1R01CA183953-01A1, R01EB014944]
Purpose: To study whether multispectral optoacoustic tomography (MSOT) can serve as a label-free imaging modality for the detection of lymph node micrometastases and in-transit metastases from melanoma on the basis of the intrinsic contrast of melanin in comparison to fluorine 18 fluorodeoxyglucose (FDG) positron emission tomography (PET)/computed tomography (CT). Materials and Methods: The study was approved by the institutional animal care and use committee. Sequential MSOT was performed in a mouse B16F10 melanoma limb lymph node metastasis model (n = 13) to survey the development of macro-, micro- and in-transit metastases (metastases that are in transit from the primary tumor site to the local nodal basin) in vivo. The in vitro limit of detection was assessed in a B16F10 cell phantom. Signal specificity was determined on the basis of a simultaneous lymphadenitis (n = 4) and 4T1 breast cancer lymph metastasis (n = 2) model. MSOT was compared with intravenous FDG PET/CT. The diagnosis was assessed with histologic examination. Differences in the signal ratio (metastatic node to contralateral limb) between the two modalities were determined with the two-tailed paired t test. Results: The mean signal ratios acquired with MSOT in micrometastases (2.5 +/- 0.3, n = 6) and in- transit metastases (8.3 +/- 5.8, n = 4) were higher than those obtained with FDG PET/CT (1.1 +/- 0.5 [ P < .01] and 1.3 +/- 0.6 [ P < .05], respectively). MSOT was able to help differentiate even small melanoma lymph node metastases from the other lymphadenopathies (P < .05 for both) in vivo, whereas FDG PET/CT could not (P > .1 for both). In vitro, the limit of detection was at an approximate cell density of five cells per microliter (P < .01). Conclusion: MSOT enabled detection of melanoma lymph node micrometastases and in-transit metastases undetectable with FDG PET/CT and helped differentiate melanoma metastasis from other lymphadenopathies. (C) RSNA, 2016
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