期刊
PURINERGIC SIGNALLING
卷 12, 期 2, 页码 283-294出版社
SPRINGER
DOI: 10.1007/s11302-016-9502-y
关键词
BDNF; GABAergic transmission; Adenosine A(2A) receptors; Hippocampus
资金
- Fundacao para a Ciencia e Tecnologia (FCT) Research Project [EXPL/BIM-MEC/0009/2013]
- FCT Fellowships [SFRH/BD/73276/2010, SFRH/BD/60386/2009, SFRH/BPD/89057/2012]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/60386/2009, SFRH/BPD/89057/2012, EXPL/BIM-MEC/0009/2013, SFRH/BD/73276/2010] Funding Source: FCT
Brain-derived neurotrophic factor (BDNF) and adenosine are widely recognized as neuromodulators of glutamatergic transmission in the adult brain. Most BDNF actions upon excitatory plasticity phenomena are under control of adenosine A(2A) receptors (A(2A)Rs). Concerning gamma-aminobutyric acid (GABA)-mediated transmission, the available information refers to the control of GABA transporters. We now focused on the influence of BDNF and the interplay with adenosine on phasic GABAergic transmission. To assess this, we evaluated evoked and spontaneous synaptic currents recorded from CA1 pyramidal cells in acute hippocampal slices from adult rat brains (6 to 10 weeks old). BDNF (10-100 ng/mL) increased miniature inhibitory postsynaptic current (mIPSC) frequency, but not amplitude, as well as increased the amplitude of inhibitory postsynaptic currents (IPSCs) evoked by afferent stimulation. The facilitatory action of BDNF upon GABAergic transmission was lost in the presence of a Trk inhibitor (K252a, 200 nM), but not upon p75(NTR) blockade (anti-p75(NTR) IgG, 50 mu g/mL). Moreover, the facilitatory action of BDNF onto GABAergic transmission was also prevented upon A(2A)R antagonism (SCH 58261, 50 nM). We conclude that BDNF facilitates GABAergic signaling at the adult hippocampus via a presynaptic mechanism that depends on TrkB and adenosine A(2A)R activation.
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