期刊
PROTEOMICS
卷 16, 期 14, 页码 1970-1974出版社
WILEY-BLACKWELL
DOI: 10.1002/pmic.201600045
关键词
Histone; Mass spectrometry; Method optimization; Propionylation; Technology
资金
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen) [SB-11179]
- BOF Mandate [01D23313]
- IWT Mandates [SB-141209]
- FWO [G013916N, Mandate 12E9716N]
Histone proteins are essential elements for DNA packaging. Moreover, the PTMs that are extremely abundant on these proteins, contribute in modeling chromatin structure and recruiting enzymes involved in gene regulation, DNA repair and chromosome condensation. This fundamental aspect, together with the epigenetic inheritance of histone PTMs, underlines the importance of having biochemical techniques for their characterization. Over the past two decades, significant improvements in mass accuracy and resolution of mass spectrometers have made LC-coupled MS the strategy of choice for accurate identification and quantification of protein PTMs. Nevertheless, in previous work we disclosed the limitations and biases of the most widely adopted sample preparation protocols for histone propionylation, required prior to bottom-up MS analysis. In this work, however, we put forward a new specific and efficient propionylation strategy by means of propionic anhydride. In this method, aspecific overpropionylation at serine (S), threonine (T) and tyrosine (Y) is reversed by adding hydroxylamine (HA). We recommend using this method for future analysis of histones through bottom-up MS.
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