期刊
PROTEOMICS
卷 16, 期 11-12, 页码 1742-1746出版社
WILEY
DOI: 10.1002/pmic.201500510
关键词
Fungal communication; MALDI MSI; Mycoparasitism; Peptaibiotics; Rhizoctonia; Trichoderma
资金
- Austrian Science Fund FWF [V139-B20]
- Vienna Science and Technology Fund [LS13-086]
- Austrian Science Fund (FWF) [V 139] Funding Source: researchfish
- Austrian Science Fund (FWF) [V139] Funding Source: Austrian Science Fund (FWF)
Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growingmedia is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized confrontation assay. By this we were able to visualize metabolite distributions by MALDI MSI after matrix deposition with a home-built sublimation device and thorough recrystallization. We present for the first time MALDI MSI data for secondary metabolite release during active mycoparasitism.
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