期刊
PROTEOMICS
卷 16, 期 11-12, 页码 1695-1706出版社
WILEY
DOI: 10.1002/pmic.201500530
关键词
Bottom-up proteomics; Electron transfer dissociation; Intact proteins; Matrix-assisted ionization; Posttranslational modifications; Technology
资金
- NSF [CAREER 0955975, CHE-1411376]
- DuPont Young Professor Award
- ASMS Research Award
- Waters Center of Innovation Program
- Eli Lilly Young Investigator Award in Analytical Chemistry
- WSU Awards from Paul and Carol Schaap
- WSU Awards from Paul and Carol Schaap (NIH) [DA011322, DA021696]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1411376] Funding Source: National Science Foundation
Matrix-assisted ionization (MAI) is a newly discovered method for converting compounds from the solid phase to gas-phase ions having charge states similar to electrospray ionization (ESI), but without the need for high-energy sources such as lasers or high voltage. Laserspray ionization (LSI) is a subset of MAI that uses a laser to provide high spatial resolution analyses, but the laser is not directly involved in the ionization process. These methods produce multiply-charged analyte ions that are useful for characterizing compounds directly from surfaces using advanced characterization technologies. Because the multiply-charged ions originate from charged matrix clusters, efficient desolvation of the matrix is a prerequisite. Here, we report on the utility of collision-induced dissociation (CID) and electron transfer dissociation (ETD) coupled to mass spectrometry using several MAI and LSI matrices for peptide and protein characterization employing mass spectrometers from two manufacturers. The information obtained is similar to that using ESI for most analyses and superior to matrix-assisted laser desorption/ionization (MALDI) as is shown for intact proteins and protein digests directly from mouse brain tissue sections. The ionization processes are soft so that posttranslational modification (e.g. phosphorylation) sites are readily determined. Instances where ETD or CID in conjunction with MAI failed are attributed to lack of desolvation of charged matrix: analyte particles.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据