期刊
PROTEIN ENGINEERING DESIGN & SELECTION
卷 29, 期 7, 页码 245-251出版社
OXFORD UNIV PRESS
DOI: 10.1093/protein/gzw015
关键词
consensus design; multiple sequence alignment; protein stability; semi-rational design; statistical sequence analysis; thermostability
资金
- Monash University
A popular and successful strategy in semi-rational design of protein stability is the use of evolutionary information encapsulated in homologous protein sequences. Consensus design is based on the hypothesis that at a given position, the respective consensus amino acid contributes more than average to the stability of the protein than non-conserved amino acids. Here, we review the consensus design approach, its theoretical underpinnings, successes, limitations and challenges, as well as providing a detailed guide to its application in protein engineering.
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