期刊
PROGRESS IN LIPID RESEARCH
卷 64, 期 -, 页码 57-68出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.plipres.2016.08.001
关键词
Lipase; Rhizopus species; Characterization; Heterologous expression; Bioengineering; Biodiesel; Enantioselectivity; sn-1,3-Position specificity
资金
- Six Talent Peaks Project in Jiangsu Province [NY-010]
- 333 Project in Jiangsu Province [BRA2015316]
- High-end Foreign Experts Recruitment Program [GDW20123200113]
- NSFC [20802027]
- National High Technology Research and Development Program of China (863 Program) [2012AA022207]
- 111 Project [111-2-06]
Lipases are versatile catalysts that hydrolyze ester bonds of water-insoluble glycerides or carry out reversible reactions at the water/lipid interface. The remarkable characteristics of lipases from the genus Rhizopus are their high sn-1,3-positional specificity, enantioselectivity and activity in nonaqueous media, which make them one of the most desirable enzymes for many applications, including lipid modification and biodiesel and chiral organic compound synthesis. sn-1,3-Position-specific Rhizopus lipases are particularly useful for the production of structured triacylglycerols. Significant progress has been made regarding lipases from the genus Rhizopus, including gene sequencing, elucidation of the protein structure and catalytic function, heterologous expression and redesigning Rhizopus lipases for valuable properties, which is receiving increasing academic and industrial attention. In this review, we present a comprehensive overview of Rhizopus lipases, focusing on (a) the characteristics of Rhizopus lipases, (b) Rhizopus lipase genes and structural features, (c) strategies for heterologous expression of Rhizopus lipase genes in yeast system, (d) progress in protein engineering for the improvement of the properties of Rhizopus lipases, and (e) development of biotechnological applications. (C) 2016 Elsevier Ltd. All rights reserved.
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