期刊
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
卷 283, 期 1825, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rspb.2016.0042
关键词
RNA interference; symbiotic bacteria; biocide; insect; Chagas disease
资金
- Biotechnology and Biological Sciences Research Council, UK, Tools and Resources Fund [BB/G024154/1]
- Grand Challenges Explorations grant from the Bill and Melinda Gates Foundation [OPP1068514]
- Genetics Society Summer Studentship
- BBSRC [BB/G024154/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G024154/1] Funding Source: researchfish
- Bill and Melinda Gates Foundation [OPP1068514] Funding Source: Bill and Melinda Gates Foundation
RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were horizontally transmissible. This represents a significant advance in the ability to deliver RNAi, potentially to a large range of non-model insects.
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