期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 113, 期 12, 页码 E1738-E1746出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1525528113
关键词
microglia; glia; developmental neuroscience; RNAseq; macrophage
资金
- National Institutes of Health (NIH) [NS069375]
- NIH
- NIH [R21HD075359, R47DA15043]
- National Research Service Award predoctoral Fellowship [F31 NS078813]
- National Research Service Award postdoctoral Fellowship [F32HL115963-02]
- T32 training Grants [5T32MH019938-22, 5K08NS075144-05]
- Australian National Health and Medical Research Council postdoctoral Fellowship [GNT1052961]
- Canadian Institutes of Health Research Fellowship
- Myelin Repair Foundation
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
- JPB Foundation
- Vincent and Stella Coates
- [S10RR025518-01]
The specific function of microglia, the tissue resident macrophages of the brain and spinal cord, has been difficult to ascertain because of a lack of tools to distinguish microglia from other immune cells, thereby limiting specific immunostaining, purification, and manipulation. Because of their unique developmental origins and predicted functions, the distinction of microglia from other myeloid cells is critically important for understanding brain development and disease; better tools would greatly facilitate studies of microglia function in the developing, adult, and injured CNS. Here, we identify transmembrane protein 119 (Tmem119), a cell-surface protein of unknown function, as a highly expressed microglia-specific marker in both mouse and human. We developed monoclonal antibodies to its intracellular and extracellular domains that enable the immunostaining of microglia in histological sections in healthy and diseased brains, as well as isolation of pure nonactivated microglia by FACS. Using our antibodies, we provide, to our knowledge, the first RNAseq profiles of highly pure mouse microglia during development and after an immune challenge. We used these to demonstrate that mouse microglia mature by the second postnatal week and to predict novel microglial functions. Together, we anticipate these resources will be valuable for the future study and understanding of microglia in health and disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据