期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 113, 期 32, 页码 9111-9116出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1604666113
关键词
DNA methylation; gene body methylation; epigenetics; histone modifications; CHROMOMETHYLASE 3
资金
- National Institutes of Health [R00GM100000]
- Pew Charitable Trusts
- Office of the Vice President of Research at UGA
- National Science Foundation (NSF) [IOS-1402183]
- NSF [MCB-0960425]
- University of Georgia (UGA)
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1402183] Funding Source: National Science Foundation
In plants, CG DNA methylation is prevalent in the transcribed regions of many constitutively expressed genes (gene body methylation; gbM), but the origin and function of gbM remain unknown. Here we report the discovery that Eutrema salsugineum has lost gbM from its genome, to our knowledge the first instance for an angiosperm. Of all known DNA methyltransferases, only CHROMOMETHYLASE 3 (CMT3) is missing from E. salsugineum. Identification of an additional angiosperm, Conringia planisiliqua, which independently lost CMT3 and gbM, supports that CMT3 is required for the establishment of gbM. Detailed analyses of gene expression, the histone variant H2A.Z, and various histone modifications in E. salsugineum and in Arabidopsis thaliana epigenetic recombinant inbred lines found no evidence in support of any role for gbM in regulating transcription or affecting the composition and modification of chromatin over evolutionary timescales.
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