期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 113, 期 35, 页码 9882-9887出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1603941113
关键词
genomics; bioenergy; biotechnological yeasts; genetic code; microbiology
资金
- Office of Science of the US DOE [DE-AC02-05CH11231]
- National Science Foundation [DEB-1442148, DEB-0072741, 0417180]
- DOE Great Lakes Bioenergy Research Center
- DOE Office of Science Grant [BER DE-FC02-07ER64494]
- US Department of Agriculture (USDA) National Institute of Food and Agriculture Hatch Project [1003258]
- European Research Council Grant [268893]
- Science Foundation Ireland Grant [13/IA/1910]
- Wellcome Trust
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [7371/13-6]
- Pew Charitable Trusts
- Alexander von Humboldt Foundation
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq
- DOE Great Lakes Bioenergy Research Center DOE Office of Science Grant [BER DE-FC02-07ER64494]
- USDA, Forest Products Laboratory
- Science Foundation Ireland (SFI) [13/IA/1910] Funding Source: Science Foundation Ireland (SFI)
- Direct For Biological Sciences
- Division Of Environmental Biology [1442113] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Division Of Environmental Biology [0417180, 1442148] Funding Source: National Science Foundation
- European Research Council (ERC) [268893] Funding Source: European Research Council (ERC)
Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Ourwell-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as L-rhamnose utilization, have patchy phylogenetic distributions. Gene clusters, with variable organization and distribution, encode many pathways of interest. Genomics can predict some biochemical traits precisely, but the genomic basis of others, such as xylose utilization, remains unresolved. Our data also provide insight into early evolution of ascomycetes. We document the loss of H3K9me2/3 heterochromatin, the origin of ascomycete mating-type switching, and panascomycete synteny at the MAT locus. These data and analyses will facilitate the engineering of efficient biosynthetic and degradative pathways and gateways for genomic manipulation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据