期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 113, 期 51, 页码 14823-14828出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1614417113
关键词
nicotinic receptor; consciousness; ultraslow fluctuations; anesthesia; prefrontal cortex
资金
- Ile-de-France Domaine d'Interet Majeur (NeRF/DIM)
- programme PasteurInnov
- Fondation de la Recherche Medicale (FRM) [SPF20140129365, DPA20140629803]
- Agence Nationale de la Recherche (ANR), ANR Neuroscience, by the Centre National de la Recherche Scientifique Unite Mixte de Recherche 3571
- Laboratoire d'Excellence LABEX BIO-PSY by the FP7 ERANET programme NICO-GENE [n009 BLANC 20092009BLANC 20]
- European Commission FP7 RTD Project [HEALTH-2009-Neurocyp.08-202088, 242167]
- French National Cancer Institute [CANCEROPOLE IDF 2016-1-TABAC-01-IP-1 MASKOS]
- European Commission Flagship, the Human Brain Project
- Stavros Niarchos Foundation
- LABEX BIO-PSY
- French state funds [ANR-11-IDEX-0004-02]
The prefrontal cortex (PFC) plays an important role in cognitive processes, including access to consciousness. The PFC receives significant cholinergic innervation and nicotinic acetylcholine receptors (nAChRs) contribute greatly to the effects of acetylcholine signaling. Using in vivo two-photon imaging of both awake and anesthetized mice, we recorded spontaneous, ongoing neuronal activity in layer II/III in the PFC of WT mice and mice deleted for different nAChR subunits. As in humans, this activity is characterized by synchronous ultraslow fluctuations and neuronal synchronicity is disrupted by light general anesthesia. Both the alpha 7 and beta 2 nAChR subunits play an important role in the generation of ultraslow fluctuations that occur to a different extent during quiet wakefulness and light general anesthesia. The beta 2 subunit is specifically required for synchronized activity patterns. Furthermore, chronic application of mecamylamine, an antagonist of nAChRs, disrupts the generation of ultraslow fluctuations. Our findings provide new insight into the ongoing spontaneous activity in the awake and anesthetized state, and the role of cholinergic neurotransmission in the orchestration of cognitive functions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据