期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 113, 期 50, 页码 14324-14329出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1608917113
关键词
stilbene; dioxygenase; structure; carotenoid
资金
- US Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231, DE-FG02-07ER64495]
- National Institutes of Health, National Institute of General Medical Sciences
- US Department of Energy [DE-AC02-05CH11231]
- National Science Foundation [1355438]
Stilbenes are diphenyl ethene compounds produced naturally in a wide variety of plant species and some bacteria. Stilbenes are also derived from lignin during kraft pulping. Stilbene cleavage oxygenases (SCOs) cleave the central double bond of stilbenes, forming two phenolic aldehydes. Here, we report the structure of an SCO. The X-ray structure of NOV1 from Novosphingobium aromaticivorans was determined in complex with its substrate resveratrol (1.89 angstrom), its product vanillin (1.75 angstrom), and without any bound ligand (1.61 angstrom). The enzyme is a seven-bladed beta-propeller with an iron cofactor coordinated by four histidines. In all three structures, dioxygen is observed bound to the iron in a side-on fashion. These structures, along with EPR analysis, allow us to propose a mechanism in which a ferric-superoxide reacts with substrate activated by deprotonation of a phenol group at position 4 of the substrate, which allows movement of electron density toward the central double bond and thus facilitates reaction with the ferric superoxide electrophile. Correspondingly, NOV1 cleaves a wide range of other stilbene-like compounds with a 4'-OH group, offering potential in processing some solubilized fragments of lignin into monomer aromatic compounds.
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