期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 113, 期 5, 页码 1214-1219出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1524607113
关键词
chromatin; nucleosome; DNA; X-ray structure; MMTV
资金
- Swiss National Fund Grant [310030B_138742]
- European Research Council Grant [322778]
- Swiss National Science Foundation (SNF) [310030B_138742] Funding Source: Swiss National Science Foundation (SNF)
- European Research Council (ERC) [322778] Funding Source: European Research Council (ERC)
The conformation of DNA bound in nucleosomes depends on the DNA sequence. Questions such as how nucleosomes are positioned and how they potentially bind sequence-dependent nuclear factors require near-atomic resolution structures of the nucleosome core containing different DNA sequences; despite this, only the DNA for two similar a-satellite sequences and a sequence (601) selected in vitro have been visualized bound in the nucleosome core. Here we report the 2.6-angstrom resolution -ray structure of a nucleosome core particle containing the DNA sequence of nucleosome A of the 3'-LTR of the mouse mammary tumor virus (147 bp MMTV-A). To our knowledge, this is the first nucleosome core particle structure containing a promoter sequence and crystallized from Mg2+ ions. It reveals sequence-dependent DNA conformations not seen previously, including kinking into the DNA major groove.
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