期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 113, 期 26, 页码 E3619-E3628出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1522631113
关键词
fragile X syndrome; FMRP; diacylglycerol kinase; CLIP; translation control
资金
- ANR [ANR-12-BSV8-0022]
- Fondation Jerome Lejeune
- College de France
- USIAS
- APLM
- [ANR-10-LABX-0030-INRT]
- [ANR-10-IDEX-0002-02]
- Agence Nationale de la Recherche (ANR) [ANR-12-BSV8-0022] Funding Source: Agence Nationale de la Recherche (ANR)
Fragile X syndrome (FXS) is caused by the absence of the Fragile X Mental Retardation Protein (FMRP) in neurons. In the mouse, the lack of FMRP is associated with an excessive translation of hundreds of neuronal proteins, notably including postsynaptic proteins. This local protein synthesis deregulation is proposed to underlie the observed defects of glutamatergic synapse maturation and function and to affect preferentially the hundreds of mRNA species that were reported to bind to FMRP. How FMRP impacts synaptic protein translation and which mRNAs are most important for the pathology remain unclear. Here we show by cross-linking immunoprecipitation in cortical neurons that FMRP is mostly associated with one unique mRNA: diacylglycerol kinase kappa (Dgk kappa), a master regulator that controls the switch between diacylglycerol and phosphatidic acid signaling pathways. The absence of FMRP in neurons abolishes group 1 metabotropic glutamate receptor-dependent DGK activity combined with a loss of Dgk kappa expression. The reduction of Dgk kappa in neurons is sufficient to cause dendritic spine abnormalities, synaptic plasticity alterations, and behavior disorders similar to those observed in the FXS mouse model. Overexpression of Dgk kappa in neurons is able to rescue the dendritic spine defects of the Fragile X Mental Retardation 1 gene KO neurons. Together, these data suggest that Dgk kappa deregulation contributes to FXS pathology and support a model where FMRP, by controlling the translation of Dgk kappa, indirectly controls synaptic proteins translation and membrane properties by impacting lipid signaling in dendritic spine.
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