期刊
CHINESE JOURNAL OF CHEMICAL ENGINEERING
卷 23, 期 1, 页码 227-233出版社
CHEMICAL INDUSTRY PRESS
DOI: 10.1016/j.cjche.2014.11.001
关键词
Cassava residue; Manganese peroxidase; Phanerochaete chrysosporium; Solid state fermentation; Indigo carmine
资金
- Science & Technology Program of Jiangsu Province [BE2011623]
- Scientific Research Project of Provincial Environmental Protection Bureau of Jiangsu Province [2012047]
Bioconversion of lignocellulosic wastes to higher value products through fungal fermentation has economic and ecological benefits. In this study, to develop an effective strategy for production of manganese peroxidase ( MnP) from cassava residue by Phanerochaete chrysosporium in solid state fermentation, the stimulators of MnP production were screened and their concentrations were optimized by one-at-a-time experiment and Box-Behnken design. The maximum MnP activity of 186.38 nkat.g(-1) dry mass of the sample was achieved after 6 days of fermentation with the supplement of 79.5 mmol.L-1.kg(-1) acetic acid, 3.21 ml.kg(-1) soybean oil, and 28.5 g.kg(-1) alkaline lignin, indicating that cassava residue is a promising substrate for MnP production in solid state fermentation. Meanwhile, in vitro decolorization of indigo carmine by the crude MnP was also carried out, attaining the ratio of 90.18% after 6 h of incubation. An oxidative mechanism of indigo carmine decolorization by MnP was proposed based on the analysis of intermediate metabolites with ultra-high performance liquid chromatography and gas chromatography tandem mass spectrometry. Using the crude MnP produced from cassava residue for indigo carmine decolorization gives an effective approach to treat dyeing effluents. (C) 2014 The Chemical Industry and Engineering Society of China, and Chemical Industry Press. All rights reserved.
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