4.2 Article

Enhanced production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer and antimicrobial yellow pigmentation from Cupriavidus sp USMAHM13 with antibiofilm capability

期刊

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
卷 47, 期 4, 页码 388-396

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2016.1252925

关键词

Antibiofilm; Cupriavidus sp; USMAHM13; P(3HB-co-4HB) copolymer; pigment-coated scaffold; yellow pigment

资金

  1. Ministry of Education, Malaysia [203/PBIOLOGI/6730102, 203/PBIOLOGI/6711357]

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Antibiofilm polymers have the ability to inhibit bacterial biofilm formation, which is known to occur ubiquitously in the environment and pose risks of infection. In this study, production of P(3HB-co-4HB) copolymer and antimicrobial yellow pigment from Cupriavidus sp. USMAHM13 are enhanced through medium optimization. Before the improvement of yellow pigment production, screening for the best additional supplement was performed resulting in high-yield yellow pigmentation using yeast extract with optimum concentration of 2g/L. Effects of different concentrations of 1,4-butanediol, ammonium acetate, and yeast extract were studied using central composite design. Under optimal conditions, 53wt% of polyhydroxyalkanoate (PHA) content, 0.35g/L of pigment concentration, and 5.87g/L of residual biomass were achieved at 0.56wt% C of 1,4-butanediol, 1.14g/L of ammonium acetate, and 2g/L of yeast extract. Antibiofilm tests revealed that the yellow pigment coated on P(3HB-co-4HB) copolymer had significant effect on the inhibition of bacteria proliferation and colonization from 6hr onward reaching 100% inhibition by 12hr, hence effectively inhibiting the biofilm formation.

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