期刊
POLYMERS FOR ADVANCED TECHNOLOGIES
卷 28, 期 7, 页码 905-908出版社
WILEY
DOI: 10.1002/pat.3990
关键词
affinity membrane; nanofiber; solution blowing; protein adsorption
资金
- China Scholarship Council (CSC)
- National Natural Science Foundation of China [51473121]
A facile spinning-based strategy was developed to fabricate chitosan (CS) surface nanofiber-based affinity membranes for protein adsorption. The core-shell nanofiber mat of nylon 6-CS was prepared via coaxial solution blowing process. The nanofibers have a diameter range of 60-300nm. The core-shell structure was confirmed by transmission electron microscopy, and CS was observed as a thin layer that uniformly adhered to the core. The dye ligand of cibacron blue F3GA (CB F3GA) was further covalently immobilized on the nanofibers with a content of 425 mu mol/g. The pristine and CB F3GA-attached mats were studied in protein adsorption. High bovine serum albumin adsorption capacities of 91.9 and 219.6mg/g were obtained for pristine and CB F3GA-attached mats, respectively. Given its properties of high flux rate and low pressure drop, CB F3GA-attached nylon 6-CS nanofiber mat meets the requirements of highly effective affinity membrane chromatography. Copyright (c) 2016 John Wiley & Sons, Ltd.
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