期刊
POLYMERS FOR ADVANCED TECHNOLOGIES
卷 28, 期 4, 页码 549-556出版社
WILEY
DOI: 10.1002/pat.3957
关键词
exopolymer; thermal properties; GC-MS; pseudoplastic; alternative substrate
资金
- CAPES [Nanobiotec - 757/09]
- Oswaldo Cruz Foundation (Fiocruz)
- Federal Institute of Bahia (IFBA)
- FAPESB
The aim of this study was to evaluate the production and properties of novel extracellular polymeric substances (EPS) from glucose (EPS 1) and crude glycerol (CG) from castor beans oil (EPS 2). Cupriavidus necator IPT 027 cultivated with glucose and CG (35 degrees C, 150rpm, 72hr, pH7, shaker) produced approximately 1.60 +/- 0.01 and 2.83 +/- 0.02gl(-1) EPS, respectively, with high-molecular weight (3.89x10(5) and 1.89x10(7)Da) and constituted of different functional groups such as uronic acid, monosaccharides (glucose, mannose, arabinose, and fucose) and primary amine group. The composition of the fermentable substrate influenced the melting temperature (221.11 and 230.18 degrees C), crystallinity (34.36% and 37.11%), degradation temperature (255.06 and 296.62 degrees C) and morphology. EPS showed pseudoplastic non-Newtonian fluid behavior in the aqueous solutions, presenting potential applicability biotechnological and industrial mainly in food industry as emulsifiers and biosurfactants. This is the first study on the production and characterization of EPS obtained by C.necator IPT 027 in culture with glucose and CG. Copyright (c) 2016 John Wiley & Sons, Ltd.
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