期刊
POLYMER CHEMISTRY
卷 7, 期 47, 页码 7242-7248出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6py01438k
关键词
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资金
- Chinese Scholarship Council for a PhD scholarship
- Ghent University (BOF)
- Humbold Foundation for a Feodor Lynen fellowship
We report on the synthesis of amine-reactive polymers, for the purpose of protein conjugation. Poly(N, N-dimethylacrylamide) (polyDMA) was prepared by RAFT polymerization using either a squaric ester amide functionalized chain transfer agent (CTA) or a phthalimide functionalized CTA which served as a precursor for post-polymerization introduction of a squaric ester amide moiety. Polymers were characterized for their end group fidelity by H-1-NMR and MALDI-TOF MS, which showed the use of a squaric ester amide functionalized CTA to be superior. The performance of these polymers for lysine-targeted protein modification was assessed using lysozyme as model protein and compared to N-hydroxysuccinimide (NHS)functionalized polymers as the golden standard in bio-conjugation. We found that squaric ester amides were much less prone to hydrolysis, which yielded higher protein-conjugation efficiency in aqueous medium.
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