期刊
POLIMEROS-CIENCIA E TECNOLOGIA
卷 26, 期 2, 页码 144-151出版社
ASSOC BRASIL POLIMEROS
DOI: 10.1590/0104-1428.2294
关键词
biopolymer; Mucor racemosus Fresenius; glycerol; aeration; fermentation; productivity
资金
- Fundacao de Amparo a pesquisa do Estado de Minas Gerais (FAPEMIG)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
This study evaluated extracellular production of biopolymer using fungus Mucor racemosus Fresenius and glycerol as a carbon source. Initially employing conical flasks of 500 mL containing 100 mL of cultive medium with 0.18 +/- 0.03 g. L-1 of microorganisms, the results showed that the best conditions of the variables studied were: initial concentration of glycerol 50 g.L-1, fermentation time of 96 h, inoculum cultivation time of 120 h, and aeration in two stages-the first 24 hours without aeration and 72 hours fermentation with aeration of 2 vvm and 2 g.L-1 of yeast extract. The experiments conducted in a Biostat B fermenter with a 2.0 L capacity that contained 1.0 L of medium showed production of 16.35 g.L-1 gum formed and 75% glycerol consumption. These conditions produced a biopolymer with the molecular weight and total sugar content of 4.607x10(6) g.mol(-1) (Da) and 89.5%, respectively.
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