期刊
PLOS ONE
卷 11, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0147229
关键词
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资金
- National Institutes of Health under Ruth L. Kirschstein National Research Service Award from the NIGMS [F32GM099291]
- National Institutes of Health [5R21CA176854-02]
- MIIE Commercialization Grant [AGR 2014-0200]
- MSF from the State of Michigan [Case-48166]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1254238] Funding Source: National Science Foundation
Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of complex genomes and haplotype phasing of mixtures of similar sequences. Here we demonstrate a method to reconstruct the sequences of individual nucleic acid molecules up to 11.6 kilobases in length from short (150-bp) reads. We show that our method can construct 99.97%-accurate synthetic reads from bacterial, plant, and animal genomic samples, full-length mRNA sequences from human cancer cell lines, and individual HIV env gene variants from a mixture. The preparation of multiple samples can be multiplexed into a single tube, further reducing effort and cost relative to competing approaches. Our approach generates sequencing libraries in three days from less than one microgram of DNA in a single-tube format without custom equipment or specialized expertise.
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