期刊
PLATELETS
卷 28, 期 3, 页码 272-280出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/09537104.2016.1218455
关键词
Microparticles; mitochondria; platelet concentrates; SPADE; storage; transfusion
资金
- Canadian Institutes of Health Research
- Canadian Blood Services
- Canadian Institutes of Health Research new investigator award
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Fond de Recherche du Quebec Nature et Technologie
- NSERC
- Health Canada
- Canadian Arthritis Network
On activation, platelets release vesicles called microparticles (MPs). MPs are heterogeneous with regard to the presence or absence of mitochondria. We quantified MPs in platelet concentrates (PCs) taking their mitochondrial content into account. Platelet-rich plasma (PRP), buffy coat (BC) and apheresis (AP) PCs were tested through 7 days of storage. A combination of flow cytometry and spanning-tree progression analysis of density-normalized events (SPADE) was used to determine MP and mitochondrial release during storage. All the PC biochemical parameters complied with transfusion standards at all times. Platelet activation markers increased during storage and were higher for PRP than other types of PCs. Concentrations of MPs and extracellular mitochondria interpreted by SPADE algorithm were significantly higher in PRP than other in PCs and were stable throughout storage. The mode of preparation, rather than storage duration, impacts the release of MPs and mitochondria in PCs.
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