期刊
PLANT SCIENCE
卷 242, 期 -, 页码 342-350出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2015.08.015
关键词
Soybean; KASPar assay; Copy Number Variation; Digital-PCR assay; Phylogenetic diversity; SCN resistance genes/QTL; SNP genotyping
资金
- United Soybean Board
- Missouri Soybean Merchandising Council
Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is a serious soybean pest. The use of resistant cultivars is an effective approach for preventing yield loss. In this study, 19,652 publicly available soybean accessions that were previously genotyped with the S0ySNP50K iSelect BeadChip were used to evaluate the phylogenetic diversity of SCN resistance genes Rhg1 and Rhg4 in an attempt to identify novel sources of resistance. The sequence information of soybean lines was utilized to develop KASPar (KBioscience Competitive Allele-Specific PCR) assays from single nucleotide polymorphisms (SNPs) of Rhgl, Rhg4, and other novel quantitative trait loci (QTL). These markers were used to genotype a diverse set of 95 soybean germplasm lines and three recombinant inbred line (RIL) populations. SNP markers from the Rhgl gene were able to differentiate copy number variation (CNV), such as resistant-high copy (PI 88788-type), low copy (Peking-type), and susceptible-single copy (Williams 82) numbers. Similarly, markers for the Rhg4 gene were able to detect Peking-type (resistance) genotypes. The phylogenetic information of SCN resistance loci from a large set of soybean accessions and the gene/QTL specific markers that were developed in this study will accelerate SCN resistance breeding programs. (C) 2015 The Authors. Published by Elsevier Ireland Ltd.
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