期刊
PLANT SCIENCE
卷 243, 期 -, 页码 56-70出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2015.11.007
关键词
Beta vulgaris ssp maritima; Sea beet; Leaf transcriptome; Salt stress; Excised leaves
资金
- National Science Center, Poland [2014/13/B/NZ9/03790]
Beta vulgaris ssp. maritima is a halophytic relative of cultivated beets. In the present work a transcriptome response to acute salt stress imposed to excised leaves of sea beet was investigated. Salt treatments consisted of adding NaCl directly to the transpiration stream by immersing the petioles of excised leaves into the salt solutions. Sequencing libraries were generated from leaves subjected to either moderate or strong salt stress. Control libraries were constructed from untreated leaves. Sequencing was performed using the Illumina MiSeq platform. We obtained 32 970 unigenes by assembling the pooled reads from all the libraries with Trinity software. Screening the nr database returned 18 362 sequences with functional annotation. Using the reference transcriptome we identified 1 246 genes that were differentially expressed after 48 h of NaCl stress. Genes related to several cellular functions such as membrane transport, osmoprotection, molecular chaperoning, redox metabolism or protein synthesis were differentially expressed in response to salt stress. The response of sea beet leaves to salt treatments was marked out by transcriptomic up-regulation of genes related to photosynthetic carbon fixation, ribosome biogenesis, cell wall-building and cell wall expansion. Furthermore, several novel and undescribed transcripts were responsive to salinity in leaves of sea beet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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