Differential heat sensitivity index in barley cultivars (Hordeum vulgare L.) monitored by chlorophyll a fluorescence OKJIP

The objective of this study was to differentiate the heat tolerance in ten varieties of barley (Hordeum vulgare L.) originating from Morocco. Five modern varieties and five landraces (local varieties) collected at five different geographical localities in the south of Morocco were investigated in the present study. After two weeks of growth, detached leaves were short term exposure to various temperatures (25, 30, 35, 40, and 45 degrees C) for 10 min in the dark. Two chlorophyll a fluorescence parameters derived from chlorophyll a fluorescence transient (OKJIP) (performance index (PIABS) and relative variable fluorescence at the K-step (V-K)) were analysed. Heat treatment had a significant effect on the PIABS and V-K at 45 degrees C treatment and the analysis of variance for PIABs and V-K is highly significant between all varieties. The slope of the relationship between logPI(ABS) and V-K named heat sensitivity index (HSI) was used to evaluate the thermotolerance of photosystem II (PSII) between the studied barley varieties. According to this approach, barley varieties were screened and ranked for improving heat tolerance. HSI was found to be a new indicator with regard to distinguishing heat tolerance of different barley cultivars. (C) 2016 Elsevier Masson SAS. All rights reserved.