期刊
PLANT PHYSIOLOGY
卷 173, 期 1, 页码 326-337出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.16.01219
关键词
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资金
- National Science Foundation [IOS-0721192, IOS-1322796, PHY-1126386, PHY-1058470]
- Research Growth Initiative at the University of Wisconsin-Milwaukee
- University of Wisconsin-Madison/University of Wisconsin-Milwaukee Intercampus Research Incentive Grants Program
- Greater Milwaukee Foundation
- University of Wisconsin-Milwaukee Research Foundation
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1322796] Funding Source: National Science Foundation
Cell signaling pathways mediated by leucine-rich repeat receptor-like kinases (LRR-RLKs) are essential for plant growth, development, and defense. The EMS1 (EXCESS MICROSPOROCYTES1) LRR-RLK and its small protein ligand TPD1 (TAPETUM DETERMINANT1) play a fundamental role in somatic and reproductive cell differentiation during early anther development in Arabidopsis (Arabidopsis thaliana). However, it is unclear whether other cell surface molecules serve as coregulators of EMS1. Here, we show that SERK1 (SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1) and SERK2 LRR-RLKs act redundantly as coregulatory and physical partners of EMS1. The SERK1/2 genes function in the same genetic pathway as EMS1 in anther development. Bimolecular fluorescence complementation, Forster resonance energy transfer, and coimmunoprecipitation approaches revealed that SERK1 interacted biochemically with EMS1. Transphosphorylation of EMS1 by SERK1 enhances EMS1 kinase activity. Among 12 in vitro autophosphorylation and transphosphorylation sites identified by tandem mass spectrometry, seven of them were found to be critical for EMS1 autophosphorylation activity. Furthermore, complementation test results suggest that phosphorylation of EMS1 is required for its function in anther development. Collectively, these data provide genetic and biochemical evidence of the interaction and phosphorylation between SERK1/2 and EMS1 in anther development.
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