期刊
Plant Pathology Journal
卷 32, 期 6, 页码 584-+出版社
KOREAN SOC PLANT PATHOLOGY
DOI: 10.5423/PPJ.NT.07.2016.0153
关键词
antifungal; Bacillus; interaction; multifactorial; Myoviridae
资金
- Research Grant of Pukyong National University
Several Bacillus species were isolated from rice field soils, and 16S rRNA gene sequence analysis showed that Bacillus cereus was the most abundant. A strain named BC1 showed antifungal activity against Rhizoctonia solani. Bacteriophages infecting strain BC1 were isolated from the same soil sample. The isolated phage PK16 had an icosahedral head of 100 +/- 5 nm and tail of 200 +/- 5 nm, indicating that it belonged to the family lifyoviridae. Analysis of the complete linear dsDNA genome revealed a 158,127-bp genome with G + C content of 39.9% comprising 235 open reading frames as well as 19 tRNA genes (including 1 pseudogene). Blastp analysis showed that the proteins encoded by the PK16 genome had the closest hits to proteins of seven different bacteriophages. A neighbor-joining phylogenetic tree based on the major capsid protein showed a robust clustering of phage PK16 with phage JIBP901 and BCP8-2 isolated from Korean fermented food.
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