期刊
PLANT JOURNAL
卷 87, 期 4, 页码 335-342出版社
WILEY-BLACKWELL
DOI: 10.1111/tpj.13186
关键词
Rauvolfia serpentina; monoterpenoid indole alkaloid; Apocynaceae; gamma-tocopherol-like N-methyltransferase family; norajmaline N-methyltransferase; ajmaline N-beta-methyltransferase
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant
- Canada Research Chairs
- Genome Canada
- Genome Alberta
- Genome Prairie
- Genome British Columbia
- Canada Foundation for Innovation
- Ontario Ministry of Research and Innovation
- National Research Council of Canada
- Consejo Nacional de Ciencia y Tecnologia (CONACyT), Mexico
Ajmaline biosynthesis in Rauvolfia serpentina has been one of the most studied monoterpenoid indole alkaloid (MIA) pathways within the plant family Apocynaceae. Detailed molecular and biochemical information on most of the steps involved in the pathway has been generated over the last 30 years. Here we report the identification, molecular cloning and functional expression in Escherichia coli of two R. serpentina cDNAs that are part of a recently discovered gamma-tocopherol-like N-methyltransferase (gamma-TLMT) family and are involved in indole and side-chain N-methylation of ajmaline. Recombinant proteins showed remarkable substrate specificity for molecules with an ajmalan-type backbone and strict regiospecific N-methylation. Furthermore, N-methyltransferase gene transcripts and enzyme activity were enriched in R. serpentina roots which correlated with accumulation of ajmaline alkaloid. This study elucidates the final step in the ajmaline biosynthetic pathway and describes the enzyme responsible for the formation of N-beta-methylajmaline, an unusual charged MIA found in R. serpentina.
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