期刊
PLANT CELL AND ENVIRONMENT
卷 39, 期 10, 页码 2097-2107出版社
WILEY
DOI: 10.1111/pce.12739
关键词
ARC; Chlamydomonas; nitric oxide; nitrite; NR; molybdenum; multidomain proteins
资金
- MINECO (Ministerio de Economia y Competitividad, Spain) [BFU2011-29338]
- European Fondo Europeo de Desarrollo Regional (FEDER) program
- Junta de Andalucia [P08-CVI-04157, BIO-128, BIO-286]
- Plan Propio de la Universidad de Cordoba
- MECD (Ministerio de Educacion, Cultura y Deporte, Spain) [AP2009-3859]
Nitric oxide (NO) is a relevant signal molecule involved in many plant processes. However, the mechanisms and proteins responsible for its synthesis are scarcely known. In most photo-synthetic organisms NO synthases have not been identified, and Nitrate Reductase (NR) has been proposed as the main enzymatic NO source, a process that in vitro is also catalysed by other molybdoenzymes. By studying transcriptional regulation, enzyme approaches, activity assays with in vitro purified proteins and in vivo and in vitro NO determinations, we have addressed the role of NR and Amidoxime Reducing Component (ARC) in the NO synthesis process. N\R and ARC were intimately related both at transcriptional and activity level. Thus, arc mutants showed high NIA1 (NR gene) expression and NR activity. Conversely, mutants without active NR displayed an increased ARC expression in nitrite medium. Our results with nia1 and arc mutants and with purified enzymes support that ARC catalyses the NO production from nitrite taking electrons from NR and not from Cytb5-1/Cytb5-Reductase, the component partners previously described for ARC (proposed as NOFNiR, Nitric Oxide-Forming Nitrite Reductase). This NR-ARC dual system would be able to produce NO in the presence of nitrate, condition under which NR is unable to do it.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据