期刊
PLANT CELL AND ENVIRONMENT
卷 39, 期 6, 页码 1304-1319出版社
WILEY
DOI: 10.1111/pce.12682
关键词
branched-chain amino acids; drought; ETF/ETFQO pathway; metabolomics; respiration; tricarboxylic acid cycle
资金
- Max Planck Society
- National Council for Scientific and Technological Development CNPq-Brazil [483525/2012-0]
- Deutsche Forschungsgemeinschaft [MA2379/7-1]
- DAAD/CAPES (Deutsche Akademische Austausch Dienst/Coordination for Scientific Support for Post-Graduate Level Training)
- FAPEMIG (Foundation for Research Assistance of the Minas Gerais State, Brazil)
- National Council for Scientific and Technological Development CNPq-Brazil, Brazil
- CNPq-Brazil
During dark-induced senescence isovaleryl-CoA dehydrogenase (IVDH) and D-2-hydroxyglutarate dehydrogenase (D-2HGDH) act as alternate electron donors to the ubiquinol pool via the electron-transfer flavoprotein/electron-transfer flavoprotein: ubiquinone oxidoreductase (ETF/ETFQO) pathway. However, the role of this pathway in response to other stresses still remains unclear. Here, we demonstrated that this alternative pathway is associated with tolerance to drought in Arabidopsis. In comparison with wild type (WT) and lines overexpressing D-2GHDH, loss-of-function etfqo-1, d2hgdh-2 and ivdh-1 mutants displayed compromised respiration rates and were more sensitive to drought. Our results demonstrated that an operational ETF/ETFQO pathway is associated with plants' ability to withstand drought and to recover growth once water becomes replete. Drought-induced metabolic reprogramming resulted in an increase in tricarboxylic acid (TCA) cycle intermediates and total amino acid levels, as well as decreases in protein, starch and nitrate contents. The enhanced levels of the branched-chain amino acids in loss-of-function mutants appear to be related to their increased utilization as substrates for the TCA cycle under water stress. Our results thus show that mitochondrial metabolism is highly active during drought stress responses and provide support for a role of alternative respiratory pathways within this response.
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