期刊
PLANT CELL
卷 28, 期 2, 页码 439-453出版社
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.15.01033
关键词
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资金
- Swiss National Science Foundation [31003A-141117/1]
- University of Geneva
- Ernest Boninchi Foundation
- Ernst and Lucie Schmidheiny Foundation
- European Molecular Biology Organization [ASTF485-2014]
- Deutsche Forschungsgemeinschaft [WE2231/8-2, EXC 1028]
Vitamin B-6 comprises a family of compounds that is essential for all organisms, most notable among which is the cofactor pyridoxal 5'-phosphate (PLP). Other forms of vitamin B-6 include pyridoxamine 5'-phosphate (PMP), pyridoxine 5'-phosphate (PNP), and the corresponding nonphosphorylated derivatives. While plants can biosynthesize PLP de novo, they also have salvage pathways that serve to interconvert the different vitamers. The selective contribution of these various pathways to cellular vitamin B-6 homeostasis in plants is not fully understood. Although biosynthesis de novo has been extensively characterized, the salvage pathways have received comparatively little attention in plants. Here, we show that the PMP/PNP oxidase PDX3 is essential for balancing B-6 vitamer levels in Arabidopsis thaliana. In the absence of PDX3, growth and development are impaired and the metabolite profile is altered. Surprisingly, RNA sequencing reveals strong induction of stress-related genes in pdx3, particularly those associated with biotic stress that coincides with an increase in salicylic acid levels. Intriguingly, exogenous ammonium rescues the growth and developmental phenotype in line with a severe reduction in nitrate reductase activity that may be due to the overaccumulation of PMP in pdx3. Our analyses demonstrate an important link between vitamin B-6 homeostasis and nitrogen metabolism.
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