期刊
PLANT CELL
卷 28, 期 8, 页码 1879-1893出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.16.00108
关键词
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资金
- National Key Basic Research Developments Program, Ministry of Science and Technology, China [2013CB126902]
- National Key Technologies Research and Development Program of China [2016YFD0100804]
- National Natural Science Foundation of China [31322040]
- National Transgenic Major Program Grants [2016ZX08009003-003-007]
- China Innovative Research Team, Ministry of Education
- Programme of Introducing Talents of Discipline to Universities (111 Project) [B14016]
- Science and Technology Commission of Shanghai Municipality [13JC1408200]
- Biotechnology and Biological Sciences Research Council [BB/N002628/1] Funding Source: researchfish
- BBSRC [BB/N002628/1] Funding Source: UKRI
F-box proteins constitute a large superfamily in plants and play important roles in controlling many biological processes, but the roles of F-box proteins in male meiosis in plants remain unclear. Here, we identify the rice (Oryza sativa) F-box gene MEIOTIC F-BOX (MOF), which is essential for male meiotic progression. MOF belongs to the FBX subfamily and is predominantly active during leptotene to pachytene of prophase I. mof meiocytes display disrupted telomere bouquet formation, impaired pairing and synapsis of homologous chromosomes, and arrested meiocytes at late prophase I, followed by apoptosis. Although normal, programmed double-stranded DNA breaks (DSBs) form in mof mutants, foci of the phosphorylated histone variant gamma H2AX, a marker for DSBs, persist in the mutant, indicating that many of the DSBs remained unrepaired. The recruitment of Completion of meiosis I (COM1) and Radiation sensitive51C (RAD51C) to DSBs is severely compromised in mutant meiocytes, indicating that MOF is crucial for DSB end-processing and repair. Further analyses showed that MOF could physically interact with the rice SKP1-like Protein1 (OSK1), indicating that MOF functions as a component of the SCF E3 ligase to regulate meiotic progression in rice. Thus, this study reveals the essential role of an F-box protein in plant meiosis and provides helpful information for elucidating the roles of the ubiquitin proteasome system in plant meiotic progression.
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