期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 14, 期 10, 页码 2010-2020出版社
WILEY
DOI: 10.1111/pbi.12560
关键词
Populus; biofuel; cell wall; xylose; lignin; recalcitrance
资金
- DOE BioEnergy Science Center project
- Office of Biological and Environmental Research in the U.S. Department of Energy Office of Science
- U.S. Department of Energy [DE-AC05-00OR22725]
- Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, U.S. Department of Energy [DE-FG02-93ER20097]
Plant laccases are thought to function in the oxidation of monolignols which leads to higher order lignin formation. Only a hand-full of laccases in plants have been functionally evaluated, and as such little is known about the breadth of their impact on cell wall chemistry or structure. Here, we describe a previously uncharacterized laccase from Populus, encoded by locus Potri.008G064000, whose reduced expression resulted in transgenic Populus trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. These phenotypes are consistent with plant biomass exhibiting reduced recalcitrance. Interestingly, the transgene effect on recalcitrance is dependent on a mild pretreatment prior to chemical extraction of sugars. Metabolite profiling suggests the transgene modulates phenolics that are associated with the cell wall structure. We propose that this particular laccase has a range of functions related to oxidation of phenolics and conjugation of flavonoids that interact with lignin in the cell wall.
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