期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 15, 期 3, 页码 297-305出版社
WILEY
DOI: 10.1111/pbi.12612
关键词
Penicillium canescens; biocatalysis; elicitation; astragaloside IV; hairy root cultures
资金
- National Key Research Development Program of China [2016YFD0600805]
- Application Technology Research and Development Program of Harbin [2013AA3BS014]
- Fundamental Research Funds for the Central Universities [2572015EA04, 2572015AA14, 2572014AA06]
- Special Fund of National Natural Science Foundation of China [31270618]
A novel biotechnology approach by combining deacetylation biocatalysis with elicitation of immobilized Penicillium canescens (IPC) in Astragalus membranaceus hairy root cultures (AMHRCs) was proposed for the elevated production of astragaloside IV (AG IV). The highest AG IV accumulation was achieved in 36-day-old AMHRCs co-cultured with IPC for 60 h, which resulted in the enhanced production of AG IV by 14.59-fold in comparison with that in control (0.193 +/- 0.007 mg/g DW). Meanwhile, AG IV precursors were almost transformed to AG IV by IPC deacetylation. Moreover, expression of genes involved in AG IV biosynthetic pathway was significantly up-regulated in response to IPC elicitation. Also, FTIR and SEM showed that cell wall lignification was enhanced following IPC treatment and root surface was likely to be IPC deacetylation site. Overall, dual roles of IPC (biocatalyst and elicitor) offered an effective and sustainable way for the mass production of AG IV in AMHRCs.
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