期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 15, 期 5, 页码 581-593出版社
WILEY
DOI: 10.1111/pbi.12655
关键词
biofuel; lignin modification; bacteria; Sphingomonas paucimobilis; Lig beta-aryl ether; saccharification yield
资金
- Carlsberg Foundation
- Villum Foundation
- VILLUM Center of Excellence 'Plant Plasticity'
- Center for Synthetic Biology 'bioSYNergy'
- UCPH Excellence Program for Interdisciplinary Research
- European Commission's Directorate-General for Research within the 7th Framework Program (FP7) [270089]
- Hercules program of Ghent University for the Synapt Q-Tof [AUGE/014]
- Agency for Innovation by Science and Technology (IWT) in Flanders
- Agency for Innovation by Science and Technology of Chile
- Research Foundation-Flanders (FWO)
- DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science) [DE-FC02-07ER64494]
- Innovation Fund Denmark [4106-00037B]
- Villum Fonden [00007523] Funding Source: researchfish
Lignin is a major polymer in the secondary plant cell wall and composed of hydrophobic interlinked hydroxyphenylpropanoid units. The presence of lignin hampers conversion of plant biomass into biofuels; plants with modified lignin are therefore being investigated for increased digestibility. The bacterium Sphingomonas paucimobilis produces lignin-degrading enzymes including LigD, LigF and LigG involved in cleaving the most abundant lignin interunit linkage, the beta-aryl ether bond. In this study, we expressed the LigD, LigF and LigG (LigDFG) genes in Arabidopsis thaliana to introduce postlignification modifications into the lignin structure. The three enzymes were targeted to the secretory pathway. Phenolic metabolite profiling and 2D HSQC NMR of the transgenic lines showed an increase in oxidized guaiacyl and syringyl units without concomitant increase in oxidized beta-aryl ether units, showing lignin bond cleavage. Saccharification yield increased significantly in transgenic lines expressing LigDFG, showing the applicability of our approach. Additional new information on substrate specificity of the LigDFG enzymes is also provided.
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