期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 15, 期 1, 页码 82-96出版社
WILEY
DOI: 10.1111/pbi.12593
关键词
moso bamboo; flower formation and development; regulatory pathway; Dof; MADS-box; microRNA
资金
- National Natural Science Foundation of China [31570673]
- National High Technology Research and Development Program of China 'Moso Bamboo Functional Genomics Research' [2013AA102607-4]
Moso bamboo is characterized by infrequent sexual reproduction and erratic flowering habit; however, the molecular biology of flower formation and development is not well studied in this species. We studied the molecular regulation mechanisms of moso bamboo development and flowering by selecting three key regulatory pathways: plant-pathogen interaction, plant hormone signal transduction and protein processing in endoplasmic reticulum at different stages of flowering in moso bamboo. We selected PheDof1, PheMADS14 and six microRNAs involved in the three pathways through KEGG pathway and cluster analysis. Subcellular localization, transcriptional activation, Western blotting, insitu hybridization and qRT-PCR were used to further investigate the expression patterns and regulatory roles of pivotal genes at different flower development stages. Differential expression patterns showed that PheDof1, PheMADS14 and six miRNAs may play vital regulatory roles in flower development and floral transition in moso bamboo. Our research paves way for further studies on metabolic regulatory networks and provides insight into the molecular regulation mechanisms of moso bamboo flowering and senescence.
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