期刊
PLANT AND SOIL
卷 414, 期 1-2, 页码 53-67出版社
SPRINGER
DOI: 10.1007/s11104-016-3106-8
关键词
Cold stress; Hydrogen; miRNA; Oryza sativa; ROS; Target genes
资金
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Natural Science Foundation of Jiangsu Province [BK20130683]
- Fundamental Research Funds for the Central Universities [KYTZ201402]
- National Natural Science Foundation of China [J1210056, J1310015]
Aims The role of hydrogen gas (H-2) in modulating cold tolerance was investigated. Methods A combination of physiological and molecular approaches was used to study the effect of H-2 on the alleviation of cold stress. Results Cold stress stimulated the production of H-2 in rice seedlings. The pretreatment with exogenous H-2 not only mimicked above physiological response, but also prevented cold-induced growth inhibition. With 0.39 mM H-2 pretreatment, rice seedlings exhibited the maximum improving responses, including alleviating the decrease of chlorophyll contents and photosynthetic activity. The lipid peroxidation and the overproduction of reactive oxygen species (ROS) were decreased as well. These results were supported by the changes of total and isozymatic activities of antioxidant enzymes. In comparison with cold stress alone, miR398 transcripts were down-regulated by H-2, and expression levels of its targets Cu/Zn-SOD1 (CSD1) and Cu/Zn-SOD2 (CSD2) were increased. By contrast, miR319 transcripts were differentially increased, showing a relatively negative correlation with its target genes PROLIFERATING CELL FACTOR5 (PCF5) and PROLIFERATING CELL FACTOR8 (PCF8). Conclusions Our results suggested that endogenous H-2 might contribute to the enhancement of cold tolerance, at least partially, by the reestablishment of redox homeostasis via miR398 and miR319.
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