期刊
PIGMENT CELL & MELANOMA RESEARCH
卷 29, 期 5, 页码 583-589出版社
WILEY-BLACKWELL
DOI: 10.1111/pcmr.12507
关键词
uveal melanoma; circulating melanoma cells; monosomy-3; dual immunomagnetic enrichment; immuno-FISH
资金
- internal research fund of the University of Lubeck, Lubeck, Germany [FC 20222]
Monosomy-3 in primary uveal melanoma (UM) is associated with a high risk of metastasis and mortality. Although circulating melanoma cells (CMC) can be found in most UM patients, only approximately 50% of the patients develop metastases. We utilized a novel immuno-FISH assay to detect chromosome-3 in intact CMC isolated by dual immunomagnetic enrichment. Circulating melanoma cells were detected in 91% of the patients (n = 44) with primary non-metastatic UM, of which 58% were positive for monosomy-3. The monosomy-3 status of CMC corresponded to the monosomy-3 status of the primary tumor in 10 of the 11 patients where this could be tested. Monosomy-3 in the CMC was associated with an advanced tumor stage (P = 0.046) and was detected in all four patients who developed metastasis within the follow-up period of 4 yr. This non-invasive technique may enable the identification of UM patients at risk for metastasis particularly when a primary tumor specimen is unavailable.
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