P450-mediated detoxification of botanicals in insects

In recent years, botanical insecticides have gradually replaced the traditional synthetic insecticides, with the properties of strong pertinence and environment friendly. Many new botanical insecticides are developed and used, meanwhile insect develop its resistance to these insecticides. Previous studies have proved that Cytochrome P450 monooxygenases (CYPs) play a critical role in insect detoxification. Genomic and proteomic approaches focused on P450 expression and regulation at the transcript and protein levels have made substantial progress. The review covers the recent years' research of typical botanicals metabolism mediated by CYPs, the relationship between function and structural characters of P450 enzymes, as well as the expression and regulation of related P450 genes in botanicals metabolism.