Alkaloidal metabolites from Aspergillus felis and their activities against Paracoccidioides brasiliensis

In this study, the fungus Aspergillus felis (strain UFMGCB 8030) was isolated from rocks in the Atacama Desert, Chile. Its CH2Cl2 extract exhibited antifungal activity (MIC of 1.9 mu g/mL) against Paracoccidioides brasiliensis (Pb18), which is the etiological agent for paracoccidioidomycosis. The crude extract was purified, and a new cytochalasin derivative, cytochalasin Z(15E) (1), and one ardeemin derivative, 5-N-acetyl-8-beta-isopropyl-ardeemin (4), in addition to five known secondary metabolites, rosellichalasin (2), cytochalasin E(3), gancidin (5), pseurotin A1 (6), and 2,4-dihydroxyacetophenone (7), were isolated. The structures of these compounds were confirmed by 1D and 2D NMR spectroscopy and HR-ESI-MS. Cytochalasin E was the most active compound with MIC an value of 3.6 mu M, while the other isolated compounds exhibited weak antifungal activities (MIC values > 100.0 mu M). (C) 2016 Phytochemical Society of Europe. Published by Elsevier Ltd. All rights reserved.