期刊
PHYSIOLOGICAL GENOMICS
卷 48, 期 8, 页码 554-564出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/physiolgenomics.00028.2016
关键词
global gene expression microarray; coronary artery disease (CAD); ADTRP; histone; cyclin D1; CDK4; p21(CIP); caspase 7
资金
- China National Natural Science Foundation Key Program [31430047]
- Chinese National Basic Research Programs (973 Programs) [2013CB531101, 2012CB517801]
- Key Project in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period [2011BAI11B19]
- Hubei Province's Outstanding Medical Academic Leader Program
- Hubei Province Natural Science Key Program [2014CFA074]
- China National Natural Science Foundation grant [91439129, NSFC-J1103514]
- National Heart, Lung, and Blood Institute [R01 HL-121358, R01 HL-126729]
- Specialized Research Fund for Doctoral Program of Higher Education from Ministry of Education
- Innovative Development of New Drugs Key Scientific Project [2011ZX09307-001-09]
The ADTRP gene encodes the androgen-dependent TFPI-regulating protein and is a susceptibility gene for contrary artery disease (CAD). We performed global gene expression profiling for ADTRP knock-down using microarrays in human HepG2 cells. Follow-up real-time RT-PCR analysis demonstrated that ADTRP knock-down regulates a diverse set of genes, including upregulation of seven histone genes, downregulation of multiple cell cycle genes (CCND1, CDK4, and CDKN1A), and upregulation of apoptosis genes (CASP7 and PDCD2) in HepG2 cells and endothelial cells. Consistently, ADTRP increases the number of S phase cells during cell cycle, promotes cell proliferation, and inhibits apoptosis. Our study provides novel insights into the function of ADTRP and biological pathways involving ADTRP, which may be involved in the pathogenesis of CAD.
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