期刊
PHARMACOGENOMICS
卷 17, 期 3, 页码 187-197出版社
FUTURE MEDICINE LTD
DOI: 10.2217/pgs.15.166
关键词
cell apoptosis; cell proliferation; microRNA-509-3p; ovarian cancer; X-linked inhibitor of apoptosis
资金
- National Natural Science Foundation of China [81101960, 81572567]
- Shenzhen Municipal Government of China [LXRY20121106142947958]
- Laboratory Opening Fund of Sun Yat-sen University [KF201329]
Aims: XIAP is upregulated in chemoresistant epithelial ovarian cancer (EOC). However, the molecular mechanism of this dysregulation remains unclear. Materials & methods: The regulation of XIAP by miR-509-3p was investigated by luciferase reporter assay, real-time PCR and immunobloting, and the roles of miR-509-3p in cellular proliferation and apoptosis were accessed through MTT and DAPI assays. Results: miR-509-3p, a downregulated miRNA in chemoresistant EOC, can directly target the XIAP via its 3'UTR. Overexpression of miR-509-3p can not only downregulate the expression of XIAP in ovarian cancer cells but also inhibit the proliferation of EOC cells and increase their sensitivity to cisplatin-induced apoptosis. Conclusions: Our data suggest that restoring certain dysregulated miRNAs to their normal levels could increase the therapeutic effects of anticancer drugs.
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