期刊
PEPTIDES
卷 80, 期 -, 页码 25-31出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2016.03.015
关键词
Allatostatin-B; Ca2+ imaging; Gryllus bimaculatus; Receptor
资金
- JSPS KAKENHI Grant from the Ministry of Education, Science, Sports, and Culture of Japan [15J07400]
- [24580157]
- Grants-in-Aid for Scientific Research [15H04609, 15J07400] Funding Source: KAKEN
A cDNA encoding allatostatin Bs (ASTBs) containing the W(X)(6)W motif was identified using a database generated by a next generation sequencer (NGS) in the two-spotted cricket, Gryllus bimaculatus. The contig sequence revealed the presence of five novel putative ASTBs (GbASTBs) in addition to GbASTBs previously identified in G. bimaculatus. MALDI-TOF MS analyses revealed the presence of these novel and previously identified GbASTBs with three missing GbASTBs. We also identified a cDNA encoding G. bimaculatus GbASTB receptor (GbASTBR) in the NGS data. Phylogenetic analysis demonstrated that this receptor was highly conserved with other insect ASTBR5, including the sex peptide receptor of Drosophila melanogaster. Calcium imaging analyses indicated that the GbASTBR heterologously expressed in HEK293 cells exhibited responses to all identified GbASTBs at a concentration range of 10(-10)-10(-5) M. (C) 2016 Elsevier Inc. All rights reserved.
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