期刊
CHEMISTRY-AN ASIAN JOURNAL
卷 10, 期 12, 页码 2682-2687出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201500765
关键词
click chemistry; oligonucleotides; RNA; siRNA; solid-phase synthesis
资金
- KAKENHI [26810083]
- Grants-in-Aid for Scientific Research [26810083] Funding Source: KAKEN
Chimeric RNA oligonucleotides with an artificial triazole linker were synthesized using solution-phase click chemistry and solid-phase automated synthesis. Scalable synthesis methods for jointing units for the chimeric structure have been developed, and after click-coupling of the jointing units with triazole linkers, a series of chimeric oligonucleotides was prepared by utilizing the well-established phosphoramidite method for the elongation. The series of chimeric 21-mer oligonucleotides that possessed the triazole linker at different strands and positions allowed for a screening study of the RNA interference to clarify the preference of the triazole modifications in small-interfering RNA molecules.
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