期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 21, 期 12, 页码 4747-4754出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201406004
关键词
amino acids; fluorescence; fluorescent probes; imaging agents; thiols
资金
- Natural Science Foundation of China [21172137, 21302114]
- Program for New Century Excellent Talents in University [NCET-11-1034]
Glutathione (GSH), the most abundant intracellular biothiol, protects cellular components from damage caused by free radicals and reactive oxygen species (ROS), and plays a crucial role in human pathologies. A fluorescent probe that can selectively sense intracellular GSH would be very valuable for understanding of its biological functions and mechanisms of diseases. In this work, a 3,4-dimethoxythiophenol-substituted coumarin-enone was exploited as a reaction-type fluorescent probe for GSH based on a chloro-functionalized coumarin-enone platform. In the probe, the 3,4-dimethoxythiophenol group functions not only as a fluorescence quencher through photoinduced electron transfer (PET) to ensure a low background fluorescence, but also as a reactive site for biothiols. The probe displays a dramatic fluorescence turn-on response toward GSH with the long-wavelength emission (600nm) and significant Stokes shift (100nm). The selectivity of the probe toward GSH over cysteine (Cys), homocysteine (Hcy), and other amino acids was demonstrated. Assisted by laser-scanning confocal microscopy, we have demonstrated that the probe could specifically sense GSH over Cys/Hcy in human renal cell carcinoma SiHa cells.
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