期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 21, 期 15, 页码 5709-5713出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201500065
关键词
arabinoxylan; carbohydrates; microarray; plant cell wall; solid-phase synthesis
资金
- Max Planck Society
- Fonds der Chemischen Industrie
- ERC
- NSF [DBI-0421683]
Monoclonal antibodies that recognize plant cell wall glycans are used for high-resolution imaging, providing important information about the structure and function of cell wall polysaccharides. To characterize the binding epitopes of these powerful molecular probes a library of eleven plant arabinoxylan oligosaccharides was produced by automated solid-phase synthesis. Modular assembly of oligoarabinoxylans from few building blocks was enabled by adding (2-naphthyl) methyl (Nap) to the toolbox of orthogonal protecting groups for solid-phase synthesis. Conjugation-ready oligosaccharides were obtained and the binding specificities of xylan-directed antibodies were determined on microarrays.
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