期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 21, 期 38, 页码 13344-13356出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201501394
关键词
dyes; pigments; fluorescence; structure-activity relationships; super-resolution microscopy; synthesis design
资金
- Bundesministerium fur Bildung und Forschung within the program Optische Technologien fur Biowissenschaften und Gesundheit [BMBF 513, FKZ 13N11066]
Far-red emitting fluorescent dyes for optical microscopy, stimulated emission depletion (STED), and ground-state depletion (GSDIM) super-resolution microscopy are presented. Fluorinated silicon-rhodamines (SiRF dyes) and phosphorylated oxazines have absorption and emission maxima at about approximate to 660 and 680nm, respectively, possess high photostability, and large fluorescence quantum yields in water. A high-yielding synthetic path to introduce three aromatic fluorine atoms and unconventional conjugation/solubilization spacers into the scaffold of a silicon-rhodamine is described. The bathochromic shift in SiRF dyes is achieved without additional fused rings or double bonds. As a result, the molecular size and molecular mass stay quite small (<600Da). The use of the =800nm STED beam instead of the commonly used one at =750-775nm provides excellent imaging performance and suppresses re-excitation of SiRF and the oxazine dyes. The photophysical properties and immunofluorescence imaging performance of these new far-red emitting dyes (photobleaching, optical resolution, and switch-off behavior) are discussed in detail and compared with those of some well-established fluorophores with similar spectral properties.
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