期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 21, 期 41, 页码 14309-14313出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201502070
关键词
dipolar cycloaddition; DNA; gold; nanostructures; photochemistry
资金
- Karlsruhe Institute of Technology (KIT) via the Helmholtz BioInterfaces in Technology and Medicine (BIFTM) program
- [GRK2039]
A photochemical approach based on nitrile imine-mediated tetrazole-ene cycloaddition is introduced to functionalize gold nanorods with biomolecules. For this purpose, a bifunctional, photoreactive linker containing thioctic acid as the Au anchoring group and a tetrazole moiety for the light-induced reaction with maleimide-capped DNA was prepared. The tetrazole-based reaction on the nanoparticles' surface results in a fluorescent pyrazoline product allowing for the spectroscopic monitoring of the reaction. This first example of nitrile imine-mediated tetrazole-ene cycloaddition (NITEC)-mediated biofunctionalization of Au nanorods paves the way for the attachment of sensitive biomolecules, such as antibodies and other proteins, under mild conditions and expands the toolbox for the tailoring of nanomaterials.
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